Variation in microsomal subfractions obtained from normal animal tissues and transformed cells following cell disruption by nitrogen cavitation |
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Authors: | Dr Ian F Pryme Asbjörn M Svardal Jon Skorve |
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Institution: | 1. Dept. of Internal Medicine, School of Medicine, University of Virginia, 22908, Charlottesville, VA, USA
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Abstract: | Summary Microsomal membranes were obtained from MPC-11 cells, L-cells, Krebs II ascites cells and various normal animal tissues following
cell disruption by nitrogen cavitation. Membrane preparations were applied to discontinuous sucrose gradients designed to
separate three fractions — heavy rough (HR), light rough (LR) and smooth (S) microsomes. In each of the transformed cell lines
all three fractions were found whilst in the normal tissues tested the HR fraction was absent. Of the normal tissues liver
and pancreas were rich in both LR and S microsomes, the presence of large amounts of LR indicating a rich protein synthesizing
activity on membrane-bound polysomes. Kidney also contained appreciable LR but much less than both liver and pancreas. Both
heart and lung contained virtually only S microsomal material — a reflection of low protein synthetic activity on membrane-bound
polysomes. Attempts to promote the appearance of the HR fraction in liver, kidney and pancreas by incubation in tissue culture
medium, or, in the case of pancreas, by cholecystokinin/pancreozymin/secretin, stimulation bothin vivo andin vitro were unsuccessful. |
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