Destruction of low efficiency markers is a slow process occurring at a heteroduplex stage of transformation |
| |
Authors: | Nadja B Shoemaker and Walter R Guild |
| |
Institution: | (1) Biochemistry Department, Duke University, Durham, North Carolina |
| |
Abstract: | Summary Direct evidence is presented that the mechanism which discriminates against low efficiency markers in transformation of Diplococcus pneumoniae of genotype hex
+ acts on them after the formation of donor-recipient heteroduplexes. This conclusion is based on assays of the transforming activity of donor markers in lysates made after various times of incubation of recipient cells following exposure to DNA. The activity of a low efficiency marker rises substantially, indicating formation of native-like heteroduplex structures, and then falls. At 37° C the process is essentially completed 10 minutes after entry, and the apparent half life of a susceptible heteroduplex is 1.5 to 2 minutes. Data from these and other experiments imply that about as many of the surviving low efficiency markers have simply escaped attack as have been inserted into both strands by the excision-repair process suggested by Ephrussi-Taylor. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|