Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture |
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Authors: | Emiko Okubo-Kurihara Takumi Higaki Yukio Kurihara Natsumaro Kutsuna Junji Yamaguchi Seiichiro Hasezawa |
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Affiliation: | (1) Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa Chiba, 277-8562, Japan;(2) Institute for Bioinformatics Research and Development (BIRD), Japan Science and Technology Agency (JST), Suita, Japan;(3) Plant Genomic Network Research Team, Plant Functional Genomics Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan;(4) Faculty of Advanced Life Science and Graduate School of Life Science, Hokkaido University, Kita-ku N10-W8, Sapporo 060-0810, Japan; |
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Abstract: | Sucrose plays an important role in several cellular processes since it is a general source of metabolic energy, serves as a precursor for starch and cellulose synthesis, and is a metabolic starting point for carboxylate- and amino acid synthesis. While plant vacuole is the main cellular storage pool, where sucrose accumulates to high concentrations, only a small number of vacuolar sugar transporters have been identified and characterized to date. We initially identified a vacuolar sucrose transporter (NtSUT4) from tobacco BY-2 cells and established transgenic tobacco BY-2 cell lines that overexpress NtSUT4-GFP (BY-SUTG cells). Using a model system for synchronous cell elongation in miniprotoplasts (evacuolated cells) prepared from tobacco BY-2 cells, we found that NtSUT4-GFP overexpression inhibited cell growth towards the cell major axis. Moreover, under the same conditions, we found that the cell walls were well stained by calcofluor in BY-SUTG cells than in wild type BY-2 cells. These results suggest that NtSUT4 is involved in cell shape via sucrose homeostasis in plant cells. |
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