| Abstract: | A variant-specific surface antigen from a cloned population of Trypanosoma brucei S42 has been isolated and partially characterized. 35S]L-methionine was found to be incorporated into this material by cells incubated in vitro in a chemically defined medium. Incorporation of 35S]L-methionine was inhibited by cycloheximide and puromycin at concentrations which are known to specifically inhibit protein synthesis in other systems. The rate of synthesis of the variant-specific antigen in vitro has been estimated to be about 8% of the rate in vivo. Newly synthesized 35S]L-methionine-labelled variant-specific antigen was incorporated into the surface coat. |
