Characterization of the N-linked glycans of Giardia intestinalis |
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Authors: | Morelle Willy Jimenez Juan-Carlos Cieniewski-Bernard Caroline Dei-Cas Eduardo Michalski Jean-Claude |
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Institution: | Unité Mixte de Recherche CNRS/USTL 8576, Glycobiologie Structurale et Fonctionnelle, IFR 118, Batiment C9, Université des Sciences et Technologies de Lille 1, 59655 Villeneuve d'Ascq Cedex, France. willy.morelle@univ-lille1.fr |
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Abstract: | This article reports the first rigorous evidence for the existence of N-glycans in Giardia intestinalis, a parasite that is a widespread human pathogen, being a major cause of enteric disease in the world. Excreted/secreted molecules of G. intestinalis are known to stimulate the immune system. Structural strategies based on MALDI and electrospray mass spectrometry were employed to examine the excreted/secreted molecules for their N-glycan content. These revealed that the major oligosaccharides released by peptide N-glycosidase F are complex-type structures and correspond to bi-, and triantennary structures without core (alpha1,6) fucosylation. The major nonreducing epitopes in these complex-type glycans are: Galbeta1-4GlcNAc (LacNAc) and NeuAc alpha2-6Galbeta1-4GlcNAc (sialylated LacNAc). |
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Keywords: | excretory and secretory products / Giardia / glycans / parasite / structure analysis |
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