Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization |
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| Authors: | Evani Viegas-Péquignot Li Zhen Lin Bernard Dutrillaux Françoise Apiou Denise Paulin |
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| Affiliation: | (1) Institut Curie, Section Biologie, URA 620 CNRS, Stucture et Mutagenèse Chromosomiques, 26, Rue d'Ulm, F-75231 Paris Cédex 05, France;(2) Laboratoire de Biologie Moléculaire de la Differentiation de l'Université de Paris 7 et de l'Institut Pasteur, 25, Rue du Dr. Roux, F-75724 Paris Cédex 15, France |
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| Abstract: | Summary A 3-kb DNA fragment, inserted in Bluescribe vector, was used to localize the desmin gene by in situ hybridization on human metaphase chromosomes. The probe was labelled by Bio-11-dUTP and detected by immunofluorescence. Subsequent R-banding indicated that the desmin gene is located in band 2q35. |
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