The nucleotide sequence of the replication control region of the resistance plasmid R1drd-19 |
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Authors: | Peter Stougaard, Sø ren Molin, Kurt Nordströ m Flemming G. Hansen |
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Affiliation: | (1) Department of Molecular Biology, Odense University, Campusvej 55, 5230 Odense M, Denmark;(2) Department of Microbiology, The Technical University of Denmark, Building 221, 2800 Lyngby, Denmark |
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Abstract: | Summary The region of plasmid R1 containing the replication control genes has been sequenced using the Maxam-Gilbert method. The nucleotide sequence of two small PstI restriction fragments (a total of about 1,000 base pairs) was determined for the wildtype R1 plasmid as well as for two different copy mutants. It was found that one copy mutant has a single base substitution in the fragment which was recently shown to harbor an important inc/cop gene (Molin and Nordström 1980). Furthermore, the sequence indicates the presence of a structural gene that codes for a polypeptide of size 10,500 daltons. Possible gene products predicted from the nucleotide sequences and their role in replication control are discussed. |
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