Isozyme gene markers in the dioecious species Asparagus officinalis L. |
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Authors: | E Maestri F M Restivo G P Marziani Longo A Falavigna F Tassi |
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Institution: | (1) Institute of Genetics, University of Parma, Viale delle Scienze, I-43100 Parma, Italy;(2) Department of Biology, General Botanical Section, C.N.R. Study Center for the Cellular and Molecular Biology of Plants, Via Celoria 26, I-20133 Milano, Italy;(3) Research Institute for Vegetable Crops, Via Paullese 28, I-20075 Montanaso Lombardo (MI), Italy |
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Abstract: | Summary Extracts from phylloclads of Asparagus officinails were electrophoretically analyzed for isozyme polymorphism. Fourteen enzyme systems were examined using four buffer systems: seven enzymes (acid phosphatase, catalase, glutamate-oxaloacetate transaminase, isocitrate dehydrogenase, malate dehydrogenase, peroxidase, and 6-phosphogluconate dehydrogenase) exhibited clear and consistent banding patterns. Isozyme polymorphism was studied in seven pairs of male and female doubled haploids and in their male F1s. Segregation of polymorphic loci was examined in the backcross progenies and was found to be consistent with a simple Mendelian inheritance in all cases, except for three anodical peroxidases, where two factors have been hypothesized. No linkage could be found between isozyme markers that were segregating in the same cross, but association was demonstrated between one malate dehydrogenase locus and the sex determining genes. The availability of isozyme markers may be useful in breeding and, in particular, the localization of one malate dehydrogenase locus on the sex chromosomes may be helpful in mapping the sex genes. |
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Keywords: | Asparagus officinalis Isoenzymes Marker genes Sex-linked inheritance |
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