美花石斛菌根真菌接菌方式与接种效应初步研究

自然条件下，兰科菌根真菌的共生对于兰科植物种子萌芽和植株生长是必不可少的。为探讨有益共生真菌对兰科植物生长的促进作用，特别是在实验室环境下接菌方式的改变与接种效应直接的联系，本研究从野生美花石斛(Dendrobium loddigesii Rolfe.)新鲜营养根中分离、筛选出3种菌根真菌（M1、M2和M3），采用单一接菌和混合接菌的接种方式，分析3个菌株及其不同接种方式对美花石斛生长的影响。研究得到优势菌株M1和M3，并证实混合接菌对美花石斛的生物量增长具有较好的正效应，两两混合接种方式M1-M2、M2-M3及3个混合接种方式M1-M2-M3均能较好的促进美花石斛生物量的积累。充分发挥混合接菌对兰科植物生长发育所产生的效能，提高生产效率，具有较强的现实意义。

Inoculation Methods and Effects of Beneficial Symbiotic Fungi of Dendrobium loddigesii Rolfe.(Orchidaceae)

Symbiosis with orchid mycorrhizal fungi is essential to the orchid seeds germination and seedlings growth in nature. To study the effects of orchid mycorrhizae on the growth of orchid seedlings, especially the direct relationship between inoculation methods and inoculation effects in laboratory, three mycorrhizal fungi (M1, M2 and M3) was isolated from fresh nutritious roots of Dendrobium loddigesii and used to establish the asymbiotic culture system, single and mixed inoculation were adopted, inoculation effects on D.loddigesii seedlings was analyzed. At last, beneficial fungi M1 and M3 were screened out, and mixed inoculating was good for biomass accumulation of D.loddigesii seedlings, such as inoculation modes with two mycorrhizal fungi M1-M2, M2-M3 and with three mycorrhizal fungi M1-M2-M3. Mixed inoculating would play an effective role in orchids production, which has great practical significance.