Protein engineering: opportunities and challenges |
| |
Authors: | Matti Leisola Ossi Turunen |
| |
Institution: | (1) Laboratory of Bioprocess Engineering, Helsinki University of Technology, P.O. Box 6100, 02015 HUT Espoo, Finland |
| |
Abstract: | The extraordinary properties of natural proteins demonstrate that life-like protein engineering is both achievable and valuable.
Rapid progress and impressive results have been made towards this goal using rational design and random techniques or a combination
of both. However, we still do not have a general theory on how to specify a structure that is suited to a target function
nor can we specify a sequence that folds to a target structure. There is also overreliance on the Darwinian blind search to
obtain practical results. In the long run, random methods cannot replace insight in constructing life-like proteins. For the
near future, however, in enzyme development, we need to rely on a combination of both. |
| |
Keywords: | Protein engineering Directed evolution Enzymes |
本文献已被 PubMed SpringerLink 等数据库收录! |
|