| Affiliation: | (1) Wageningen Centre for Food Sciences, Diedenweg 20, 6700, AN, Wageningen, The Netherlands;(2) TNO Quality of Life, Utrechtseweg 48, 3700, AJ, Zeist, The Netherlands;(3) BioDetection Systems, Kruislaan 406, 1098, SM, Amsterdam, The Netherlands;(4) Department of Agrotechnology and Food Sciences, Wageningen University and Research Centre, Systems and Control Group, Bornsesteeg 59, 6708, PD, Wageningen, The Netherlands;(5) HAS Den Bosch, Onderwijsboulevard 221, 5200, MA, Den Bosch, The Netherlands |
| Abstract: | Background Clone-based microarrays, on which each spot represents a random genomic fragment, are a good alternative to open reading frame-based microarrays, especially for microorganisms for which the complete genome sequence is not available. Since the generation of a genomic DNA library is a random process, it is beforehand uncertain which genes are represented. Nevertheless, the genome coverage of such an array, which depends on different variables like the insert size and the number of clones in the library, can be predicted by mathematical approaches. When applying the classical formulas that determine the probability that a certain sequence is represented in a DNA library at the nucleotide level, massive amounts of clones would be necessary to obtain a proper coverage of the genome. |
