杂草稻叶绿体籼粳分化的多重PCR分析

通过分析籼稻93-11和粳稻培矮64S的叶绿体全基因组,优化和构建了籼粳分化的叶绿体分子标记ORF100和ORF29-TrnC^GCA的多重PCR。应用这个多重PCR对200余份世界各地杂草稻和其它水稻材料进行分析。结果表明:杂草稻中有明显的叶绿体籼粳分化,表现出明显的地域性,且与传统的中国栽培稻的南籼北粳能较好的对应。推测粳型杂草稻可能是栽培稻突变或粳型水稻(作母本)与其它类型水稻材料杂交而形成的。

Multiplex PCR analysis of Indica-japonica differentiation of the chloroplast DNA in weedy rice

The whole chloroplast genomes of the Indica cultivar 93-11 and Japonica cultivar Peiai64S were analyzed and compared. A multiplex PCR marker of the chloroplast DNA ORF100 and ORF29-TrnC^GCA region was optimized and constructed. More than 200 weedy rice,Asian cultivated rice and common wild rice accessions were then tested using the multiplex PCR. The results showed that there were obvious chloroplast DNA indica-japonica differentiations amongst the weedy,Asian cultivated and common wild rice accessions. Furthermore,the differentiations in weedy rice were correlated to the collected regions that were in accordance with the south indica type and north japonica type of cultivated rice in China. It was suggested that the japonica line of weedy rice should evolve from degraded cultivated rice or the japonica varieties(as the female parent)natural hybridization with other oryza materials for there was no wild form in north China.