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Sequence analysis of 16S rRNA genes amplified from two ribosomal RNA gene clusters of Bifidobacterium bifidum |
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| Authors: | Jung-Hoon Yoon Dong Koo Yim Michael Goodfellow Yong-Ha Park |
| Institution: | (1) Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Yusong, Taejon, 305-600, Korea;(2) Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne, NE1 7RU, United Kingdom |
| Abstract: | Two rRNA gene clusters were detected in the genome of Bifidobacterium bifidum KCTC 3202T using Southern blot analysis. To analyse the sequences of the 16S rRNA genes from rrnA and rrnB, 16S rDNAs were amplified by PCR using DNA fragments purified from gel slices containing each of the rRNA gene clusters. The amplified 16S rDNAs from rrnA and rrnB were cloned into vectors and three clones of each gene sequenced. The resultant sequences were confirmed by direct sequencing of the 16S rDNAs from rrnA and rrnB. Sequence differences were not found between rrnA and rrnB in 1488 bp of the 16S rRNA genes. |
| Keywords: | Bifidobacterium bifidum rRNA operon 16S rDNA |
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