Effect of seed sludge microbial community and activity on the performance of anaerobic reactors during the start-up period |
| |
Authors: | N A Oz O Ince G Turker B K Ince |
| |
Institution: | (1) Department of Environmental Engineering, Faculty of Engineering and Architecture, Canakkale Onsekiz Mart University, Terzioglu Campus, Canakkale, Turkey;(2) Department of Environmental Engineering, Istanbul Technical University, 34469 Istanbul, Turkey;(3) Institute of Environmental Sciences, Bogazici University, Bebek, 34342 Istanbul, Turkey |
| |
Abstract: | In this study, two laboratory-scale anaerobic batch reactors started up with different inoculum sludges and fed with the same
synthetic wastewater were monitored in terms of performance and microbial community shift by denaturant gradient gel electrophoresis
fingerprinting and subsequent cloning, sequencing analysis in order to reveal importance of initial quality of inoculum sludge
for operation of anaerobic reactors. For this purpose, two different seed sludge were evaluated. In Reactor1 seeded with a
sludge having less diverse microbial community (19 operational taxonomic unit (OTU’s) for Bacterial and 8 OTU’s for Archaeal
community, respectively) and a methanogenic activity of 150 ml CH4 g TVS−1 day−1, a chemical oxygen demand (COD) removal efficiency of 78.8 ± 4.17% was obtained at a substrate to microorganism (S/X) ratio
of 0.38. On the other hand, Reactor2, seeded with a sludge having a much more diverse microbial community (24 OTU’s for Bacterial
and 9 OTU’s for Archaeal communities, respectively) and a methanogenic activity, 450 ml CH4 g TVS−1 day−1, operated in the same conditions showed a better start-up performance; a COD removal efficiency of over 98% at a S/X ratio
of 0.53. Sequence analysis of Seed2 revealed the presence of diverse fermentative and syntrophic bacteria, whereas excised
bands of Seed1 related to fermentative and sulfate/metal-reducing bacteria. This study revealed that a higher degree of bacterial
diversity, especially the presence of syntrophic bacteria besides the abundance of key species such as methanogenic Archaea
may play an important role in the performance of anaerobic reactors during the start-up period. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|