A lipase preparation with dual pH optima,wide temperature optima and broad substrate specificity for multiple applications |
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| Authors: | C. M. Upadhyay P. N. Nehete R. M. Rothari |
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| Affiliation: | (1) Industrial Fermentation Division, Sarabhai Research Centre, 390 007 Baroda, (India);(2) Present address: Synbiotics Limited, 390 007 Baroda, (India) |
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| Abstract: | Summary Optimised procedures for the production and recovery of lipase at 25 litre scale by submerged fermentation using aRhizopus species isolated from petroleum-soaked soil are developed. These lipase preparations are characterized by dual pH optima (4.0 and 8.0), wide temperature optima (over 80% activity between 40–57°C), broad specificity for substrates, high degree of tolerance for common salts and excellent stability during usage as well as storage. |
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