Isolation of angiotensin-converting enzyme inhibitor from tuna muscle |
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| Authors: | Y Kohama S Matsumoto H Oka T Teramoto M Okabe T Mimura |
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| Affiliation: | Faculty of Pharmaceutical Sciences, Osaka University, Japan. |
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| Abstract: | A novel inhibitor of angiotensin-converting enzyme (ACE) has been discovered and isolated in a pure form from acid extract of tuna muscle by successive column chromatographies and HPLC. The final preparation showed IC50 values of 1 microM and 2 microM for ACEs from bovine and rabbit lungs, respectively. The amino acid sequence of the inhibitor has been established as Pro-Thr-His-Ile-Lys-Trp-Gly-Asp by the Edman procedure and carboxypeptidase digestion. |
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