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A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

Authors:	Email author" target="_blank">Rajiv?Tyagi Email author Richard?Lai Ronald?G?Duggleby

Institution:	(1) Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane Qld, 4072, Australia

Abstract:	Background Site-directed mutagenesis is an efficient method to alter the structure and function of genes. Here we report a rapid and efficient megaprimer-based polymerase chain reaction (PCR) mutagenesis strategy that by-passes any intermediate purification of DNA between two rounds of PCR.

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