The isolation of mitochondria from the colourless alga Prototheca zopfii |
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| Authors: | D Lloyd |
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| Affiliation: | 1. Department of Medical Biochemistry, Semmelweis University, Budapest 1094, Hungary;2. MTA-SE Lendület Hereditary Endocrine Tumours Research Group, Budapest 1088, Hungary;3. MTA-SE Lendület Neurobiochemistry Research Group, Budapest 1094, Hungary;1. Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Republic of Korea;2. Institute of Health and Environment, Seoul National University, Republic of Korea;1. Research Institute of Environmental Science, College of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China;2. State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, China;3. Environmental Protection Monitoring Center Station of Zhengzhou, Zhengzhou 450007, China;4. College of Chemistry, Zhengzhou University, Zhengzhou 450001, China;1. European Molecular Biology Laboratory (EMBL), Genome Biology Unit, Meyerhofstraße 1, 69117 Heidelberg, Germany |
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| Abstract: | Methods for the preparation of mitochondria from Prototheca have been investigated and the state of these organelles prepared by various means compared by electron microscopy and measurement of succinoxidase activity. Ubiquinone and cytochrome (a+a3), b and c were present. The mitochondria oxidized NADH and succinate, and mammalian cytochrome c would act as an electron acceptor in the system. The P:O ratios for various tricarboxylic acid cycle intermediates and related metabolites were determined. |
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