Development and Utilization of VHH Antibodies Derived from Camelus Dromedarius Against Foot-and-Mouth Disease Virus |
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Authors: | Lipsa Dash Saravanan Subramaniam Sagar A. Khulape Bikash Ranjan Prusty Kamal Pargai Shirish D. Narnaware |
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Affiliation: | 1. ICAR-Directorate on Foot and Mouth Disease, Mukteswar, Nainital, Uttarakhand, India;2. ICAR-National Research Center on Camel, Jorbeer, Bikaner, Rajasthan, India |
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Abstract: | Foot-and-mouth disease (FMD) is an acute, highly contagious, and economically devastating viral disease of domestic and wildlife species. For effective implementation of FMD control program, there is an imperative need for developing a rapid, sensitive, and specific diagnostics which help in the identification of serotypes involved in the outbreaks. The humoral immune response of the Camelidae is unique since in these animals 75% of circulating antibodies are constituted by heavy-chain antibodies and 25% are conventional immunoglobulin with two identical heavy chains. In the present study, we developed and characterized FMD virus-specific single-domain heavy-chain antibodies (VHHs) against inactivated whole-virus antigens of FMDV serotypes O (INDR2/1975), A (IND40/2000), and Asia 1 (IND63/1972) vaccine strains. After six rounds of panning and enrichment, these VHHs were stably expressed in Escherichia coli cells. The VHHs directed against outer capsid proteins of FMD virus were successfully utilized as the capture antibody in liquid-phase blocking ELISA (LPBE) thus replacing rabbit coating antibodies. Our study demonstrated the utility of FMD virus-specific VHHs as potential candidates in FMD research and diagnostic application. |
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Keywords: | ELISA FMDV heavy-chain antibodies immune library |
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