Polymorphic Microsatellite Markers for Solen grandis and Their Cross-Species Amplification in Three Other Species |
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Authors: | Hongtao Nie Liwen Jiang Yijie Gao Hong Xu Shangkun Du |
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Institution: | 1. College of Fisheries and Life Science, Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian Ocean University, Dalian, China;2. Ocean and Fisheries Science Research Institute of Jinzhou, Jinzhou, China |
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Abstract: | The grand jackknife clam Solen grandis is a commercially important mollusk species, but has been suffering from severe population decline due to over-exploitation and habitat destruction in China. To promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. In this study, 10 novel polymorphic microsatellite makers were developed and characterized from the S. grandis through high throughput sequencing. The number of alleles at each locus ranged from 10 to 34 with an average of 20.8 alleles per locus. The observed and expected heterozygosities varied from 0.433 to 1.000 and from 0.696 to 0.976, with an average of 0.793 and 0.884, respectively. The polymorphism information content (PIC) value ranged from 0.633 (Sg43838) to 0.958 (Sg3754), with an average of 0.858. The cross-species amplification transferability of 10 loci to three closely related species ranged from 4.17 to 62.5%. These microsatellite loci will be useful for further investigation of population structure and conversation genetics of this species. |
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Keywords: | Cross-species genetic diversity microsatellite polymorphism Solen grandis |
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