Inhibitory effect of Ocotea quixos (Lam.) Kosterm. and Piper aduncum L. essential oils from Ecuador on West Nile virus infection |
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| Authors: | Matteo Radice Agostina Pietrantoni Alessandra Guerrini Massimo Tacchini Gianni Sacchetti Matteo Chiurato |
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| Institution: | 1. Department of Earth Sciences, Universidad Estatal Amazónica, Puyo, Ecuador;2. National Center for Innovative Technologies in Public Health, Istituto Superiore di Sanità, Rome, Italy;3. Department of Life Sciences and Biotechnology (SVeB), University of Ferrara, Malborghetto di Boara, Ferrara, Italy |
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| Abstract: | West Nile virus (WNV) is a mosquito-borne flavivirus responsible of neuroinvasive manifestations. Natural products are well-known for their biological activities and pharmaceutical application. In this study, the inhibitory effects of essential oils (EOs) of Ocotea quixos (Lam.) Kosterm. and Piper aduncum L. on WNV replication were investigated. WNV was incubated with EOs before adsorption on Vero cells, viral replication was carried out in the absence or presence of EO. Cells were exposed to EO before the adsorption of untreated-virus. GC-MS and GC-FID were used for chemical characterization of EOs. Cell protection from infection was observed for both EOs. P. aduncum EO was characterized by dillapiole as main compound (48.21%) and O. quixos EO by 1,8-cineole (39.15%). Further investigations, such as the study of molecular and cellular mechanisms of action and in vivo evaluation, should be performed on these essential oils to derive new potential drugs against WNV. |
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| Keywords: | Ocotea quixos Piper aduncum essential oil West Nile virus antiviral activity Ecuadorian Amazon region |
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