Proteomics-driven identification of putative AfsR2-target proteins stimulating antibiotic biosynthesis inStreptomyces lividans |
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Authors: | Chang-Young Kim Hyun-Joo Park Eung-Soo Kim |
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Institution: | (1) Department of Biological Engineering, Inha University, 402-751 Incheon, Korea;(2) SongWon Envichem Inc., Kangnam-gu, 135-010 Seoul, Korea |
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Abstract: | AfsR2, originally identified fromStreptomyces lividans, is a global regulatory protein which stimulates antibiotic biosynthesis. Through its stable chromosomal integration, the
high level of gene expression ofafsR2 significantly induced antibiotic production as well as the sporulation ofS. lividans, implying the presence of yet-uncharacterized AfsR2-target proteins. To identify and evaluate the putative AfsR2-target proteins
involved in antibiotic regulation, the proteomics-driven approach was applied to the wild-typeS. lividans and theafsR2-integrated actinorhodin overproducing strain. The 2D gel-electrophoresis gave approximately 340 protein spots showing different
protein expression patterns between these twoS. lividans strains. Further MALDI-TOF analysis revealed several AfsR2-target proteins, including glyceraldehyde-3-phosphate dehydrogenase,
putative phosphate transport system regulator, guanosine pentaphosphate synthetase/polyribonucleotide nucleotidyltransferase,
and superoxide dismutase, which suggests that the AfsR2 should be a pleiotropic regulatory protein which controls differential
expressions of various kinds of genes inStreptomyces species. |
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Keywords: | AfsR2 proteomics antibiotic regulation Streptomyces |
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