Uptake of isolated plant chromosomes by plant protoplasts |
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Authors: | L Szabados Gy Hadlaczky D Dudits |
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Institution: | (1) Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary;(2) Present address: Sugar-beet Research Institute, Sopronhorpacs, Hungary |
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Abstract: | For mass isolation of plant metaphase chromosomes, cultured cells of wheat (Triticum monococcum) and parsley (Petroselinum hortense) were synchronized by hydroxyurea and colchicine treatment. This synchronization procedure resulted in high mitotic synchrony, especially in suspension cultures of parsley in which 80% of the cells were found to be at the metaphase stage. Mitotic protoplasts isolated from these synchronized cell cultures served as a source for isolation of chromosomes. The described isolation and purification method yielded relatively pure chromosome suspension. The uptake of the isolated plant chromosomes into recipient wheat, parsley, and maize protoplasts was induced by polyethylene-glycol treatment. Cytological studies provided evidences for uptake of plant chromosomes into plant protoplasts.Abbreviations PEG
polyethylene glycol
- HU
hydroxyruea
- C
colchicine
- HUC
hydroxyurea and colchicine
- CIM
chromosome isolation medium
- TCM
Tris chromosome medium |
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Keywords: | Chromosome isolation Petroselinum Polyethylene-glycol Protoplast (uptake of chromosomes) Triticum Zea |
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