Influence of pulsing and postpulsing media tonicity on electrotransformation of intact yeast cells |
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| Authors: | Valentina Ganeva Bojidar Galutzov |
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| Institution: | Laboratoire de Microbiologie, Facultéde Médecine Xavier Bichat, UniversitéParis 7, Paris, France |
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| Abstract: | Abstract The carboxylesterases from Proteus vulgaris, Salmonella enterica and Citrobacter amalonaticus were purified 104-, 95- and 120-fold, respectively by chromatography. The enzymes had similar catalytic activities but differed considerably in their inactivation by heat, di-isopropyl fluorophosphate and Cd2+, Zn2+, Hg2+ and Cu2+. Quantitative neutralization of hydrolytic activity with specific immunoglobulins indicated that the three enzymes were antigenically distinct. |
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| Keywords: | Carboxylesterase Proteus vulgaris Salmonella enterica Citrobacter amalonaticus |
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