5-methylcytosine content in the vertebrate deoxyribonucleic acids: Species specificity |
| |
| Authors: | Kothari R. M. Shankar V. |
| |
| Affiliation: | (1) Department of Zoology and Department of Chemistry, University of Poona, Poona-7, India;(2) Present address: Department of Environmental Biosciences, Naval Medical Research Inst., National Naval Medical Center, 20014 Bethesda, Maryland, USA;(3) Present address: Department of Biochemistry, College of Physicians and Surgeons Columbia University, 10032 New York, New York, USA |
| |
| Abstract: | Summary RNA-free native DNA samples, isolated by four methods, from different vertebrate tissues and species, were hydrolyzed chemically and enzymatically and analyzed by paper chromatography to estimate the base composition. It was noted that (i) all the DNA preparations analyzed contained 5-methylcytosine, (ii) on the basis of mole percent of 5-methylcytosine, the composition of DNA varied in different species, but not so much in different tissues of the same species, (iii) the method of DNA hydrolysis, but not the method of deproteinization, affected the mole percent of 5-methylcytosine, and (iv) no 5-hydroxymethylcytosine (5-HMC) was detected in any of the DNA preparations analyzed. |
| |
| Keywords: | Vertebrate Tissue DNA Base Analysis Paper Chromatography 5-Methylcytosine Content |
| 本文献已被 SpringerLink 等数据库收录！ |
|
