Detection of Shiga toxins,intimin and enterohemolysin in Escherichia coli strains isolated from children in eastern Slovakia |
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| Authors: | Liptáková A Siegfried L Podracká L Sabol M Sehnálková H Bogyiová E Rosocha J Kmetová M Kerestesová H Kotulová D |
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| Institution: | (1) Institute of Medical Microbiology, Medical Faculty, P.J. Šafárik University, Košice, Slovakia;(2) Pediatric Clinic, Faculty Hospital, Košice, Slovakia;(3) Department of Microbiology, Faculty Hospital, Košice, Slovakia;(4) Associated Tissue Bank, Medical Faculty, P.J. Šafárik University, Košice, Slovakia;(5) Microbiological Institute, Medical Faculty, Komensky University, Bratislava, Slovakia |
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| Abstract: | FiftyEscherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with
diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined
for the presence of Shiga toxin-producingE. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination
were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin;
genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence ofE. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains)
was demonstrated. |
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