In vitro conservation of Cedrela fissilis Vellozo (Meliaceae),a native tree of the Brazilian Atlantic Forest |
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Authors: | Eduardo Da Costa Nunes Erica E Benson Ana Carla Oltramari Patrícia Sibila Araujo Juliana Righetto Moser Ana Maria Viana |
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Institution: | (1) Natural Resources Canada, Canadian Forest Service—Atlantic Forestry Centre, 1350 Regent St. South, Fredericton, NB, E3B 5P7, Canada |
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Abstract: | Two in vitro conservation methods have beendeveloped for the ex situ conservation of germplasm fromCedrela fissilis, an economically important tree of theBrazilian Atlantic Forest. The first method involves the medium-term storage, at 25°C, of artificial seeds comprising alginate encapsulatedvegetative propagules (shoot tips, cotyledonary and epicotyl nodal segments).Maximum post-storage (3 months) viabilities of 96–100% wereachieved for encapsulated shoot tips and cotyledonary nodal segments stored onwater-solidified agar (at 0.4–0.7% w/v). Encapsulated shoot tips storedfor 6 months on 0.4% (w/v) agar showed the highest survival rates(44%). Seeds of C. fissilis were successfully cryopreserved(100%) after direct immersion in liquid nitrogen. Ex situstorage procedures are now available for the medium- to long-term conservationof C. fissilis. These approaches offer new opportunitiesfor the conservation, sustainable management and utilization of this valuablefast growing timber tree. |
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Keywords: | Alginate bead encapsulation Axillary bud Germplasm conservation Plant retrieval Seed cryopreservation Shoot apices |
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