Direct Evidence for Mendelian Inheritance of the Variations in the Ribosomal Protein Gene Introns in Yellowfin Tuna (Thunnus albacares) |
| |
| Authors: | S Chow VP Scholey A Nakazawa D Margulies JB Wexler RJ Olson K Hazama |
| |
| Institution: | (1) National Research Institute of Far Seas Fisheries, Shimizu 424-8633, Japan, JP;(2) Achotines Laboratory, Las Tablas, Provincia Los Santos, Republic of Panama, PA;(3) Inter-American Tropical Tuna Commission, 8604 La Jolla Shores Drive, La Jolla, CA 92037, U.S.A., US;(4) Overseas Fishery Cooperation Foundation, Akasaka 2-17-22, Tokyo 107-0052, Japan, JP;(5) Japan Gene Research, Komagoe-naka, Shimizu 424-0904, Japan, JP |
| |
| Abstract: | Restriction fragment length polymorphism found in the S7 ribosomal protein gene introns of yellowfin tuna (Thunnus albacares) was compared between a single pair of parents and their offspring. The sizes of the first intron (RP1) and second intron (RP2) amplified by polymerase chain reaction were 810 bp and 1400 bp, respectively. The dam and sire had different restriction
types from one another in HhaI and RsaI digestions for RP1 and in DdeI, HhaI, and ScrFI digestions for RP2. Putative genotypes in both introns of 64 larvae were found to be segregated in Mendelian proportions. Genotype distributions
in a wild yellowfin tuna sample (n= 34) were in Hardy-Weinberg proportions, and observed heterozygosity ranged from 0.149 to 0.388. This study presents novel
Mendelian markers, which are feasible for tuna population genetic study and pedigree analysis.
Received December 8, 1999; accepted April 11, 2000. |
| |
| Keywords: | : EPIC-PCR ribosomal protein gene intron RFLP yellowfin tuna Mendelian marker |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
