|
|||||
|
|
Nucleolar apparatus in the macronucleus of Didinium nasutum (Ciliata): EM and 3D reconstruction |
|
| Authors: | Leonova Olga G Karajan Bella P Ivlev Yuri F Ivanova Julia L Popenko Vladimir I |
| Institution: | aEngelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991 Moscow, Russia bInstitute of Cytology, Russian Academy of Sciences, Tikhoretsky av. 4, 194064 St. Petersburg, Russia cSevertsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskij prosp. 33, 119071 Moscow, Russia |
| Abstract: | The three-dimensional (3D) organization of nucleoli in the somatic nuclei (macronuclei) of recently fed and starved Didinium nasutum was reconstructed on the basis of serial ultra-thin sections. It was shown that nucleoli, looking on the single sections like individual separate structures, appeared to be parts of the large complicated branchy nucleolar networks. A 30 h starvation did not lead to disintegration of this network, but stimulated formation of numerous vacuoles in the granular component of nucleoli, which becomes more condensed. Unlike starved D. nasutum, in fed ciliates numerous holes appeared in the fibrillar component located at the periphery of nucleoli. These holes may presumably serve as channels for transporting newly synthesized rRNA. To our knowledge, this is the first report of a 3D reconstruction of the nucleolar apparatus in ciliates. |
| Keywords: | 3D reconstruction nucleoli ciliates macronucleus |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |