Ectopic production in serum-free media of the common alpha subunit of the glycoprotein hormones |
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Authors: | Jay S Morrow Bruce C Weintraub Saul W Rosen |
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Institution: | (1) Clinical Endocrinology Branch, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Building 10, Room 8N315, 20205 Bethesda, Maryland |
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Abstract: | Summary The HeLa-S3 cell strain grown in Ham's F12 medium supplemented with insulin, transferrin, cortisol, epidermal growth factor, fibroblast
growth factor, and trace elements, but containing no serum, continued to produce the common α-subunit of the glycoprotein
hormones for the 10 d study. The amounts of α-subunit secreted into the medium during the first 4 d were indistinguishable
from those in F12 medium supplemented with 10% fetal bovine serum. During the remainder of the experiment the amounts of α-subunit
reached 50 to 80% those in the serum-supplemented medium. |
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Keywords: | HeLa ectopic α -subunit serum-free medium |
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