Organogenesis and plant formation from cotyledon explant cultures of wild turnip rape (Brassica tournefortii L.) |
| |
Authors: | R. K. Jain J. B. Chowdhury W. Friedt |
| |
Affiliation: | (1) Institut für Pflanzenbau und Pflanzenzüchtung, Justus-Liebig-Universität Giessen, D-6300 Giessen, FRG;(2) Plant Tissue Culture Lab., Department of Genetics, Haryana Agricultural University, 125 004 Hisar, India |
| |
Abstract: | Cotyledon explants of Brassica tournefortii L. were excised from germinated seedlings and cultured on Murashige & Skoog's [6] basal medium supplemented with various combinations of cytokinins and auxins, Both cytokinin and auxin were required for induction of shoot organogenesis. Of the three cytokinins tested (in combination with a low concentration of IAA), kinetin was found to be the best for shoot regeneration. On this medium, cotyledonary explants invariably underwent callusing followed by multiple shoot formation. NAA in combination with any of the three cytokinins yielded a reduced number of shoots or none, but favoured good callus growth. Callus so produced also regenerated shoots when subcultured on media containing high concentration of KIN or ZEA and low concentration of IAA. Shoots were rooted during prolonged incubation on the same medium or on MS medium free of growth regulators. Mature plants were grown in the greenhouse. |
| |
Keywords: | Brassica tournefortii tissue culture regeneration micropropagation organogenesis in vitro |
本文献已被 SpringerLink 等数据库收录! |
|