肌苷促进脑损伤后神经功能恢复的实验研究

目的：中枢损伤是目前致残率最高的疾病之一，肌苷对于神经损伤后功能恢复的促进作用已经成为研究热点，本研究拟建立一侧前肢瘫痪的大鼠脑外伤模型，证实肌苷治疗促进中枢损伤后上肢功能恢复的有效性，同时初步探索其机制。方法：建立一侧运动皮层冲击损毁的大鼠模型，通过肢体不对称实验、抓取实验等行为学观察证实其惠侧上肢功能受损，后在实验组进行肌苷药物14天，观察28天内上肢功能的恢复情况，与对照组作对比。证实其行为学上的有效性，同时对损伤侧大脑进行顺行BDA染色，探索其内在机制。结果：通过28天的观察发现经过肌苷治疗的的实验组大鼠肢体不对称实验、抓取实验等行为学评分明显好于隐性对照组，顺行BDA染色证实其有促进损伤周围健存皮层突触再生和代偿的作用。结论：肌苷可以促进中枢损伤后大鼠残存神经元得突触再生，使其大脑能在最大程度上代偿其丧失的功能，该药物可能会成为一种新的中枢损伤治疗的前体药物。

Inosine promotes recovery of neural function after traumatic brain injury

Objective： Central neurologic injury is one of the most common causes of limbs disturbance. Nowadays inosine treat- ment becomes a new focus. In this study we use a rat model of traumatic brain injury to certify the curative effect of inosine and investi- gate the internal mechanism. Methods： We established a rat model of traumatic brain injury and check the functional use of forepaw by limb use dependent test and reaching test. Thus a 14d term of inosine treatment was applied to the experimental group. All the rats expe- rienced a 28d follow-up. Both the behavior test and BDA tracing technique was used for exploring the curative effect of inosine and its internal mechanism. Results： After a 14d treatment of inosine we found that the fimctional use of the affect limb in experimental group were much better than the traumatic brain injury rat without treatment in the control group. BDA tracing technique demonstrated that the synaptic regeneration of the intact neurons around the injury cortex may contribute to the motor recovery. Conclusion： Inosine couls pro- mote synaptic regeneration of the intact neurons around the injury cortex in the traumatic brain injury rat and let the brain regain the con- trol of the paralyzed limb, It could develop as a new drug for the treatment of central neurologic injury.