流式细胞术BrdU／DNA双染法测定云芝糖肽诱导的Molt-4细胞周期阻滞

目的：探究云芝糖）Ik（PSP）对人急性淋巴母细胞白血病Molt-4细胞周期的影响。方法：采用流式细胞术BrdU／DNA双染法获得各时相细胞分布状况和细胞周期的动力学参数。结果：0．1mg／mlPSP处理12h后，G2／M期细胞百分比由对照组的11．09％减少至3．69％。DNA合成时间由12．10h延长至108．40h。24h处理组中，S期细胞百分比由对照组的43.29％增加至67．26％，而G0／G1期和G2／M期细胞百分比均减少，G0／G1期细胞百分比由对照组的37．47％减少至27．43％，G2／M期细胞百分比由对照组的19．24％降低至5.31％。DNA合成时间更是由11．95h延长至114．52h。结论：PSP对人急性淋巴母细胞白血病Molt-4细胞周期的阻滞作用在于S期．该作用与DNA合成抑制有关。

Polysaccharopeptides Derived from Trametes Versicolor Induce Cell Cycle Retardarce on Molt-4 Cells Determined by Flow Cytometry BrdU/DNA

Objective： To investigate the cell cycle inhibition effects of Polysaccharopeptides derived from Trametes versicolor （PSP） on human acute leukemia Molt-4. Methods： Flow cytometry BrdU/DNA bivariate immunostaining method was used to detect the distribution of each phase and cell cycle kinetic parameters. Results： After treatment by 0.1 mg/ml PSP for 12 h, the percentage of G2/M phase cells were dropped from 11.09% （control） to 3.69%. DNA synthesis time extended from 12.10 h to 108.40 h. In 24 h treatment group, the percentage of S phase cells increased from 43.29 %（control） to 67.26 %, while the percentage of G0/G1 phase and G2/M phase cells were all reduced, G0/G1 phase cells decreased from 37.47 % （control） to 27.43 %, G2/M phase cells reduced from 19.24 % （control） to 5.31%. DNA synthesis time was prolonged from 11.95 h to 114.52 h. Conclusions： PSP induce cell cycle retardarce in S phase on human leukemia Molt-4 cells,and related to inhibition of DNA synthesis.