内皮祖细胞移植加速鼠颈动脉内皮修复预防再狭窄的研究

目的：以安慰机组作对照，观察EPCs自体移植防治再狭窄的疗效以及该作用与血管再内皮化是否相关。方法：共30只雄性SD大鼠纳入实验，随机均分为假手术组、安慰剂组和EPCs移植组。造模前3周时取血3毫升，M199培养基培养传代，行EPCs鉴定，移植组在球囊损伤后即予尾静脉注射1×10^6个EPCs自体移植。4周后观察再狭窄和再内皮化程度。结果：与安慰剂组相比，EPCs自体移植组再内皮化程度提高，再狭窄程度减轻；EPCs移植组内膜／中膜比值与再内皮化率呈线性负相关。结论：EPCs体外扩增自体移植可预防再狭窄；其机制与加速再内皮化有关。

Effects of Autologous EPCs Transplantation on Reendothelialization and Restenosis of Ballon-injured Rat Carotis

Objective： To investigate the effects of autologous EPCs transplantation on reendothelialization and restenosis of ballon-injured rat carotis. Methods： 30 SD rats were randomly divided to equal 3 groups： sham operation group, placebo group and autologous EPCs transplantation group. In the latter two groups each rat＇s left carotid was injured by balloon. Three weeks before balloon injure, all rats were drawn 3 ml blood, and total mononuclear cells isolated from peripheral blood by density gradient centrifugation were cul- tured for three weeks. EPCs were characterized as adherent cells double positive for Dil-acLDL-uptaking and lectin-binding. Immediately after balloon impairment,and 1 × 10^6 EPCs were autologously transplanted by caudal vein injention. 4 weeks after balloon injury, the restenosis ratio and reendothelialization ratio of each subject was measured. Results： Compared with placebo group, autologous EPCs transplantation not only increased the reendothelialization ratio but also alleviated the restenosis ratio. In EPCs transplantation group, restenosis ratio was inversely linearly correlated to reendothelialization ratio. Conclusions： Autologous EPCs transdplantation can inhibit restenosis by accelerating endothelial recovery.