豚鼠皮肤光过敏试验方法的建立

目的探索并建立豚鼠光过敏试验的方法。方法采用TBS作为阳性对照物,同时设立阴性对照组。分别于第1天、第4天、第7天对豚鼠皮肤进行UV-A紫外光诱导,诱导剂量紫外光强度为30 J/cm2;于第一次诱导后28 d对豚鼠皮肤采用UV-A紫外光激发,激发强度为9 J/cm2。结果阴性对照组24 h、48 h、及72 h豚鼠光过敏试验阳性率均为0,阳性对照组为24 h、48 h、及72 h豚鼠光过敏试验阳性率均为100%。结论本研究成功建立了豚鼠皮肤光过敏试验模型,TBS可作为阳性对照物。

Establishment of the Skin Photosensitization Study Method in Guinea Pigs

Objective To explore and establish the method for the skin photosensitization in guinea pigs.Methods TBS was used as the positive control,and the Acetone as the negtive control.The shaved skin of the guinea pigs was irradiated with UV-A radiation of 30 J/cm2 repeated every 2 days for 3 consencutive days.After 4 weeks of initial sensitization,the skin of the guinea pigs was irradiated with UV-A radiation of 9 J/cm2.Results Positive ratio of the negtive control 24h,48h and 72h after irridiating was 0,and the positive control was 100%.Conclusions The skin photosensitization model was established successfully,and the TBS used as the positive control was available.