骨髓间质干细胞移植治疗大鼠脑出血模型的实验研究

目的：研究大鼠骨髓间质干细胞（Mscs）移植治疗脑出血的可行性。方法：分离MSCs后，连续传代培养、扩增，Brdu标记的MSCs通过颈动脉、侧脑室2种途径移植入脑出血大鼠模型体内，采用爬行计分法评估神经功能的恢复程度，并观察脑部Brdu阳性细胞的分布。结果：通过侧脑室、颈动脉移植后大鼠神经功能改善，明显优于对照组（P〈0．05）；经颈动脉注射组较经侧脑室注射组爬杆实验评分低（P〈0．05）。移植的MSCs主要迁移到出血灶、大脑皮层、海马区等处。结论：MSCs移植对脑出血具有保护作用，而经颈动脉给药疗效优于经侧脑室给药。

ExperimentalStudy of Bone Marrow Stem Cells Administration in Rats with Intracerebral Hemorrhage

Objective： To explore the feasibility of the rat bone marrow stromal cells （MSCs） transplantation to treat intracerebral hemorrhage. Method： MSCs were separated from rat bone marrow and cultured with culture medium. The MSCs were purified by culture expanded in vitro and then labeled with Brdu. MSCs were transferred into rat through carotid artery and lateral ventricle. The rat neurologic dysfunction ameliorating was observed by behavior scores, and distribution of Brdu labeled cells in cerebra was studied. Result： The test score of carotid artery group was lower than that of lateral ventricle group （P〈 0.05）. In the carotid artery group, more Brdu labeled cells survived or migrated. The Transferred MSCs were mainly migrated to area of hippoeampus and hemorrhage. They were also differentiated into nerve cells. Conclusion： MSCs transferring into rat brain following intracerebral hemorrhage is effective. Intra-arterial administration is more efficient.