H5N1禽流感病毒实验室检测方法灵敏性比较

目的比对实验室常用的H5N1禽流感病毒检测方法灵敏性。方法增殖H5N1病毒,蚀斑技术定量待检病毒,应用细胞接种、血凝实验、RT-PCR、Real-time RT-PCR等方法检测稀释的病毒悬液,比较检测的最低滴度。结果细胞接种、Real-time RT-PCR、RT-PCR及血凝实验能检测病毒的最低滴度为10 PFU/mL、10 PFU/mL、103 PFU/mL及3.52×105 PFU/mL。结论几种检测方法比较而言,细胞接种与Real-time RT-PCR检测灵敏性最高;血凝实验用时最短,但灵敏性低,结果需要进一步确认;RT-PCR用时较较短,检测灵敏性较高。

Sensitivity Comparision of Methods for Detection of H5N1 Influenza Virus

Objective To compare the sensitivity of methods for detecting H5N1 influenza virus.Methods The H5N1 influenza virus was amplified and quantified by plaque technology.Then the virus was diluted to compare the minimal titer using methods of cell inoculation,hemagglutinin,RT-PCR and Real-time PCR.Results The minimal titer detected using the methods of cell inoculation,hemagglutinin,RT-PCR and Real-time PCR were 10 PFU/mL,10 PFU/mL,103 PFU/mL and 3.52×105 PFU/mL seperately.Conclusions We found that the methods of cell inoculation and real-time RT-PCR were the most sensitive methods;while the hemagglutinin method was timesaving,it showed low sensitivity which need to be identified in future;RT-PCR method was timesaving,in addition,it also showed high sensitivity.