吸烟对大鼠肺组织B7-1/B7-2及其相关配体表达的影响

目的通过研究吸烟对大鼠肺组织B7-1/B7-2及其相关配体表达的影响,探讨专职抗原提呈细胞（APC）在吸烟所致肺部慢性炎症发生发展中的作用。方法将30只健康雄性Wistar大鼠随机分为不吸烟组、吸烟6周组和吸烟12周组,每组10只。采用免疫组化半定量法测定大鼠气道周围肺间质中慢性炎症细胞胞膜B7-1、B7-2、CD28和CTLA-4的表达水平。结果吸烟6周组与吸烟12周组大鼠肺组织B7-1、B7-2、CD28和CTLA-4表达量较不吸烟组均显著增高（P〈0.01）,吸烟12周组较吸烟6周组表达量也均增高（P〈0.01）,随吸烟时间的延长各指标表达量均呈上升趋势。结论吸烟可引起大鼠肺组织B7/CD28/CTLA-4表达水平的增高,提示APC可能在吸烟所致肺部慢性炎症发生发展中起重要作用。

The Effects of Smoking on the Expression of B7-1/B7-2 and Its Associated Ligands in the Lung Tissue of Rats

Objective To explore the roles of antigen presenting cells（APC） in the lung of chronic inflammation by investigating the effects of smoking on the expression of B7-1/B7-2 and its associated ligand in the lung tissue of rats.Methods Wistar rats（n=30） were randomly divided into non smoking group and smoking 6 weeks group,12weeks group,the number of each group was ten.Immunohistochemistry was used to semi-quantitatively analysis the changes of B7-1/B7-2 and its associated ligands expression on the cytomembrane of chronic inflammation cells which were on the pulmonary interstitium around airways of rats.Results The expression of B7-1,B7-2,CD28 and CTLA-4 in the lung tissue of the smoking 6 weeks and 12 weeks groups＇ rats were higher than those of nonsmoking group（P〈0.01）,with the smoking 12 weeks group higher than the smoking 6 weeks group（P〈 0.01）.Their expressions were increased in time-dependent manner.Conclusions Smoking can induce increasing expression of B7-1/ B7-2 and its related ligands in the lung tissue of rats;the APC may play an important role in the process of chronic pulmonary inflammation.