上肢精准测试系统在食蟹猴脑缺血研究中的应用

目的使用上肢精准测试系统对脊髓间充质干细胞治疗脑缺血的疗效进行行为学分析评价。方法成年食蟹猴18只,手术前2个月用上肢精准测试系统对动物进行训练并采集本底数据。采用光化学法制作局部脑缺血模型,造模后4周将动物随机分为对照组、干细胞治疗高剂量组和低剂量组,分别在缺血灶周围注射生理盐水、脊髓间充质干细胞5×106/只和1×106/只,造模后1 d、3 d、1、2、3、4周及干细胞注射后1d、3 d、1、2、3、4、5、6、8周采集行为学实验数据。比较造模前后和干细胞注射前后动物受损伤侧上肢抓取水果块的时间,结合神经功能评分评价造模和干细胞治疗效果。结果 18只动物手术后均出现与梗死部位对应的行为学症状。在术后采集数据的各个时间点损伤侧上肢行为学数据与手术前差异均存在极显著性（P〈0.01）。神经功能评分术后24 h最高,随后出现渐进性恢复。干细胞治疗后3 d到治疗后第8周,模型组动物与高、低剂量组动物间精准上肢运动测试结果差异均存在显著性（P〈0.05）。神经功能评分在治疗2周后出现显著性差异（P〈0.05）。高、低剂量组间差异未见显著性。结论上肢精准测试系统能客观准确的反应动物神经功能,在非人灵长类脑卒中动物模型建立和药效学评价方面有广泛应用前景。

Application of the Upper Extremity Precision Movement Analysis System in the Stroke Study in Cynomolgus Models

Objective To use the upper extremity precision movement analysis system in evaluation of the effect of human bone marrow mesenehymal stem cells（hBMSC） in the treatment of non-human primate models of photochemically-induced stroke.Methods Eighteen adult male cynomolgus monkeys were used in this study.Training the animals for 2 months with mMAP and collect the basic data before the surgery.The infarction models were induced photochemically.the18 monkeys were divided into three groups at 4 weeks after the surgery.The control group consisted of 6 animals receiving physiological saline in a volume of 250 μL at 3 mm away from the outer edge of the infarction.The high-dose and low-dose groups received hBMSC with the cell number of 5×106 and 1×106 cells/animal,respectively,at the same site and volume for the control group.Behavioral data were collected at 1,3 days,1,2,3,4 weeks after surgery and 1,3 days,1,2,3,4,5,6,8 weeks after hBMSC transplantation.A primate neurological deficit scale was used to assess the neurological deficit at the same time.Results All 18 animals showed focal neurological signs corresponding to the targeted infarct site after the surgery.The time at that the animals picked up the food was much slower than that before the surgery（P0.01）.The score was 32.3±5.13 at the first post-operatively evaluation.The average score declined but never fully recovered after 4 weeks.From the 3rd day after transplantation,there were significant differences between the control and both low and high dose-groups with the movement analysis system（P 0.05）.From the 2nd week after transplantation,there were significant differences between the control and both low and high dose-groups in neurological deficit scores（P 0.05）.There was no significant difference between the high-dose and low-dose groups.Conclusions The precision movement analysis system used in this study reflects neurological function objectively and accurately,and can be used to measure the neuronal loss in vivo and to evaluate the effects of therapeutic strategies involving neural or stem cell transplantation in the establishment of animal stroke models and evaluation of drug efficacy studies.