脑挫裂伤致miR-9升高促进神经干细胞分化

目的：探讨颅脑损伤后miR-9表达的变化和对神经干细胞分化和增值的影响，为颅脑损伤后神经功能修复治疗提出新的思路。方法：通过RT-PCR技术检测miR-9在挫裂伤脑组织中的表达情况；培养胚胎来源神经干细胞，并通过免疫荧光鉴定神经干细胞及其分化；转染miR-9后，通过MTT测定神经干细胞的增殖情况，和流式细胞仪检测分化神经元所占比例。结果：miR-9在挫裂伤脑组织中表达显著上升。对神经干细胞过表达miR-9可显著促进细胞增殖，并诱导分化成神经元。结论：脑挫裂伤时miR-9显著升高，并具有着促进神经干细胞增值和诱导分化的作用，可为伤后神经功能修复提供新的治疗方法。

Up-regulation of miR-9 Regulates Adult Neural Stem Cell Proliferation and Differentiation in Traumatic Brain Injury

Objective： To investigate the expression of miR-9 in traumatic brain injury and the impact of miR-9 for proliferation and differentiation of neural stem cells; to propose new ideas for neurological functions repair in traumatic brain injury. Methods： The expression of miR-9 was detected by RT-PCR in traumatic brain tissues; the embryonic neural stem cells were cultured and identified by immunofluorescence; After miR-9 transfection, the proliferation of neural stem cells were measured by MTT, and the proportion of differentiated neurons were measured by flow cytometry. Results： The expression ofmiR-9 was significantly up-regulated in traumatic brain injury. Overexpressing miR-9 can significantly promote neural stem cells proliferation and induced them to differentiate into neurons. Conclusion： Overexpressing of miR-9 could promote neural stern cell proliferation and induce differentiation, that provide new treatments for the restoration of neurological function after traumatic brain injury.