F344大鼠常见非增殖性病变病理学观察

目的建立F344大鼠自发性非增殖性病变背景数据,为药物安全性评价提供基础。方法 SPF级F344大鼠336只,雌雄各半,8周龄,本中心屏障环境下饲养,随机分为4个组,分别于6、12、18和24个月4个时间点处死动物32、64、64、176只,常规病理学取材制片后显微镜检查,观察统计四个时间点动物自发性病变的病变类型和发病率。结果主要的非增殖性病变包括乳头肌纤维化、肺细支气管旁慢性炎症、肾脏病变包括肾小管扩张、蛋白管型、肾小管萎缩和钙沉积等,睾丸精子生成障碍、卵巢生长卵泡数量减少等生殖器官组织的退行性改变等。结论在本中心现有条件下,随动物生存期的延长,F344大鼠自发性非增殖性疾病病变程度和发病率均有所增加。本实验丰富了F344大鼠自发性病变的背景资料,为药物安全性评价提供基础。

Pathological Observation at Common Non-proliferative Spontaneous Lesions of F344 Rats

Objective To establish the background database of non-proliferative spontaneous lesions of F344 rats for drug safety evaluation.Methods SPF F344 rats 336,male and female in half,8 months old,kept under the center barrier,were randomly divided into four groups,animals were killed32,64,64,176 at respectively 6,12,18 and 24 months time points.Tissues were examined under microscopic,and statistics 4 time points observed spontaneous animal disease type and disease incidence.Results Non-proliferative lesion consists of murine progressive cardiomyopathy,BALT,tubular lesions,and genital tissue degeneration such as reduction of ovarian cells or inhibition of spermatogenesis.Conclusions Under the condition of center barrier,the incidence of non-proliferative lesions of F344 rat increased.The present study enriched the existing F344 rat pathology background information,and laid the foundation for carrying out drug safety evaluation and research.