| 诱导表达p27对HEK293细胞生长代谢的影响 |
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| 引用本文： | 叶玲玲,刘红,李世崇,刘兴茂,陈昭烈.诱导表达p27对HEK293细胞生长代谢的影响[J].生物磁学,2011(21):4001-4004. |
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| 作者姓名： | 叶玲玲 刘红 李世崇 刘兴茂 陈昭烈 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京100071 |
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| 基金项目： | 国家”重大新药创制”科技重大专项（No.2009ZX09503-011） |
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| 摘 要： | 目的：研究诱导表达p27对HEK293细胞生长和代谢的影响。方法：将pTet—on载体和响应于Dox的p27诱导表达载体共转染HEK293细胞，随机挑选单克隆细胞株。以细胞周期分布和活细胞密度为主要观察指标，考察稳定转染的细胞在Dox诱导下的细胞生长；以Qglc、Qlac和Qgln为主要观察指标，考察转染细胞在Dox诱导下的细胞代谢。结：p27基因的表达使HEK293细胞的增殖速度显著降低，G1期细胞比例显著升高，葡萄糖消耗和乳酸生产减少。结论：诱导表达p27基因是对HEK293细胞进行G1期阻滞的一种有效策略。
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| 关 键 词： | HEK293细胞 细胞周期 代谢 p27 诱导表达 |
Effects of p27 Inducible-Expression on the Growth and Metabolism of HEK293 cells |
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| YE Ling-ling,LIU Hong,LI Shi-chong,LIU Xing-mao,CHEN Zhao-lie.Effects of p27 Inducible-Expression on the Growth and Metabolism of HEK293 cells[J].Biomagnetism,2011(21):4001-4004. |
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| Authors: | YE Ling-ling LIU Hong LI Shi-chong LIU Xing-mao CHEN Zhao-lie |
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| Institution: | (Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China) |
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| Abstract: | Objective： To investigate the effect of p27 inducible-expression on the growth and metabolism of HEK293 cells. Methods： The vector pTet-on and Dox-responsive p27-expressing vector were co-transfected into HEK293 cells and individual clones were isolated randomly. Cell growth and metabolism were assessed by cell-cycle distribution and viable cell density, Qglc, Qlac and Qgln as indexes, respectively. Results： The expression of p27 increased percentage of G1 cells, reduced consumption of glucose and production of lactate of HEK293 cells. Conclusions： The inducible-expression of p27 is an effective strategy for Gl-phase arrest of HEK293 cells. |
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| Keywords: | HEK293 cells Cell cycle Metabolism p27 Inducible-expression |
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