SFRP4抑表达对HT-29细胞侵袭能力的影响

目的：观察SFRP4对HT29细胞侵袭转移能力的影响。方法：以HT29细胞为研究对象，SFRP4siRNA通过脂质体转染HT29细胞，westernblot检测HT29细胞中wnt、细胞核β—Catenin蛋白的表达、elisa法检测细胞培养上清MMP-2、MMP．9的含量，Transwell小室观察成的转移侵袭能力。结果：干扰SFRP4后能显著降低HT29细胞中Wnt、细胞核中B—Catenin蛋白的表达、细胞培养上清MMP-2、MMP-9的含量，（P〈0．01），降低HT29细胞的转移侵袭能力（P〈0．01）。结论：抑制SFRP4表达能抑制HT29细胞的转移侵袭，其机制可能与抑制HT29细胞wnt信号通路有关。

The Effect of SFRP4 on Invasive Ability of HT-29

Objective： To study the effect of SFRP4 on invasive ability of HT29. Methods： HT29 cells were divide into control, non sense, SFRP4 siRNA groups. The effects of SFRP4 siRNA on HT-29 cells invasive ability in vitro was measured by Boyden chamber invasive models. Contents of Wnt, β -Catenin, MMP-2, MMP-9 protein were detected by western blot and ELISA methods, respectively. Results： In SFRP4 siRNA group, contents of Wnt, β -Catenin,MMP-2 and MMP-9 protein were decreased markedly （P〈0.01）, the invasive ability was decreased markedly（P〈0.01）, too. Conclusion： SFRP4 could increase the invasive ability of HT-29 cell.