普罗布考对糖尿病大鼠肾组织氧化应激的影响

目的研究普罗布考（Probucol）对糖尿病大鼠肾组织氧化应激的影响。方法采用腹腔注射链脲佐菌素（STZ）建立糖尿病大鼠模型。30只Wistar大鼠分为正常对照组（NC）、糖尿病组（DM）、糖尿病普罗布考治疗组（DP）。8周末称取体重、肾重、计算肾肥大指数（肾重/体重）,检测尿白蛋白排泄率（UAER）;测定各组生化指标包括血糖（BG）、胆固醇（TC）、三酰甘油（TG）、血清肌酐（SCr）、血尿素氮（BUN）;检测肾组织中丙二醛（MDA）的含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）与谷胱甘肽过氧化物酶（GSH-Px）活性;肾组织切片行PAS染色分析肾小球面积及肾小球体积。结果 DM组大鼠肾重、肾重/体重、UAER、TC、TG、SCr、BUN、肾小球面积、肾小球体积较NC组均明显增加,DP组上述改变较DM组均明显减轻（P〈0.05）。DP组肾组织中MDA含量明显低于DM组,SOD、CAT、GSH-Px活性明显高于DM组（P〈0.05）。结论普罗布考可能部分通过减轻肾组织氧化应激反应实现对糖尿病大鼠肾脏的保护作用。

Effect of probucol on the oxidative stress of kidney in diabetic rats

Objective To study the effect of probucol on the oxidative stress of kidney in diabetic rats.Methods Diabetes mellitus rat model was induced by streptozotocin（STZ）.30 Wistar rats were randomly divided into three groups： normal control group（NC）,diabetes mellitus group（DM）,probucol-treated diabetes mellitus group（DP）.After 8 weeks,body weight and kidney weight（calculate kidney weight / body weight）,urine albumin excretion rate（UAER） and biochemical indicators including blood glucose（BG）,total cholesterol（TC）,triglycerides（TG）,serum creatinine（SCr）,blood urea nitrogen（BUN） were measured;The levels of maleic dialdehyde（MDA）as well as the activities of antioxidant enzymes including superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-Px） in the renal tissue were determined;the renal tissue was observed by light microscopy： renal morphology parameter including glomerular area and glomerular volume by PAS staining.Results Kidney weight,ratio of kidney weight to body weight,UAER,TC,TG,SCr,BUN and glomerular area,glomerular volume on histological examination of the kidney were significantly increased in DM group rats than those in NC group.The changes described above were attenuated by treatment with probucol（P〈 0.05）.Elevated MDA level as well as decreased activities of SOD,CAT and GSH-Px in diabetic renal tissue were also remitted by probucol（P〈 0.05）.Conclusions The renoprotective mechanism of probucol may be at least partly correlated with relieving oxidative stress in the renal tissue of diabetic rats.