Preliminary crystallographic study of glycosylated recombinant human renin |
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| Authors: | L W Lim R A Stegeman N K Leimgruber J K Gierse S S Abdel-Meguid |
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| Institution: | Corporate Research and Development, Monsanto, Chesterfield Village, MO 63198. |
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| Abstract: | Single crystals of glycosylated recombinant human renin have been obtained using the hanging-drop vapor diffusion method with polyethylene glycol and sodium chloride as coprecipitants. The crystals belong to the cubic space group P2(1)3 with a = 143.0 A and contain two molecules of renin in the asymmetric unit. A self-rotation function study using 5.5 A data shows the orientation of a non-crystallographic 2-fold axis relating these two monomers. |
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