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Expression of a synthetic human growth hormone gene in yeast |
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| Authors: | T Tokunaga S Iwai H Gomi K Kodama E Ohtsuka M Ikehara O Chisaka K Matsubara |
| Affiliation: | 1. Faculty of Pharmaceutical Sciences (Tel. 06-877-5111), Osaka University, 1-6 Yamadaoka, Suita, Osaka 565 Japan;1. Institute for Cellular and Molecular Biology (Tel. 06-877-5244), Osaka University, 1-6 Yamadaoka, Suita, Osaka 565 Japan |
| Abstract: | A synthetic human growth hormone (hGH) gene was efficiently expressed under the control of the repressible acid phosphatase promoter in yeast (Saccharomyces cerevisiae). More than 10(6) molecules of hormone were formed per cell despite the fact that the gene was constructed with codon preference for Escherichia coli. |
| Keywords: | Recombinant DNA codon usage repressible acid phosphatase promoter signal sequence Ap ampicillin APase acid phosphatase C.B.B. Coomassie brilliant blue hGH human growth hormone PAGE polyacrylamide gel electrophoresis PMSF phenyl methyl sulfonyl fluoride resistance SDS sodium dodecyl sulfate [] designates plasmid-carrier state |
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