Exogenous Putrescine Reduces Sodium and Chloride Accumulation in NaCl-Treated Calli of the Salt-Sensitive Rice Cultivar I Kong Pao |
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Authors: | A Ndayiragije S Lutts |
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Institution: | (1) Unité de Biologie végétale, Université catholique de Louvain, 5 (Bte13) Place Croix du Sud, 1348 Louvain-la-Neuve, Belgium |
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Abstract: | In order to gain information on the putative involvement of polyamines (PAs) in the response of rice cells to salinity, mature
embryo-derived calli issued from the salt-sensitive cultivar I Kong Pao were exposed for 3 months to the simultaneous presence
of NaCl (0, 150 and 300 mM) and exogenous polyamines (putrescine (Put): 1 and 10 mM; spermidine (Spd): 1 and 10 mM; spermine
(Spm): 1 mM). Callus growth, endogenous PAs, Na+, K+ and Cl− concentrations were quantified and analysed in relation to cell viability based on 2,3,5-triphenytetrazolium chloride (TTC)
reduction. All exogenous PAs were efficiently absorbed from the external medium. Exogenous Put 1 mM clearly stimulated growth
of salt-stressed calli in relation to a decrease in both Na+ and Cl− accumulation. In contrast, Spd 10 mM and Spm 1 mM exacerbated the deleterious impact of NaCl on callus growth and induced
a decrease in K+ concentration. While Put helped in the maintenance of cell viability, Spd 10 mM and Spm 1 mM decreased cell viability, mainly
in relation to an inhibition of the alternative respiratory pathway. It is proposed that Put may assume positive functions
in salt stress resistance in rice. |
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Keywords: | Alternative oxidase Cell viability Mineral nutrition Oryza sativa Polyamine Salinity Triphenytetrazolium chloride |
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