Ribonucleotide reductase activity during the cell cycle of Saccharomyces cerevisiae |
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Authors: | M Lowdon E Vitols |
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Affiliation: | Chemical Research Department, Hoffmann-La Roche Inc., Nutley, New Jersey 07110 U.S.A. |
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Abstract: | A fluorometric amino acid analyzer using fluorescamine for the assay of the full array of natural amino acids including proline on a single column is reported. The proline determination was carried out by specific introduction of a solution of N-chlorosuccinimide into the flow system. Single column fluorometric amino acid analysis was carried out in a significantly shorter time and with a sensitivity almost two orders of magnitude greater than that obtained with a commerical colorimetric ninhydrin amino acid analyzer. |
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