Ribonucleotide reductase activity during the cell cycle of Saccharomyces cerevisiae

A fluorometric amino acid analyzer using fluorescamine for the assay of the full array of natural amino acids including proline on a single column is reported. The proline determination was carried out by specific introduction of a solution of N-chlorosuccinimide into the flow system. Single column fluorometric amino acid analysis was carried out in a significantly shorter time and with a sensitivity almost two orders of magnitude greater than that obtained with a commerical colorimetric ninhydrin amino acid analyzer.