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Characterization of a symbiotic,heterocystous, N2-fixing cyanobacterium fromCycas coralloid roots
Authors:A Sharma  D P Mishra  A Kumar
Institution:(1) Department of Biochemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, 263 145 Pantnagar, Uttar Pradesh, India;(2) the Centre of Advanced Studies in Botany, Banaras Hindu University, Varanasi, India
Abstract:A symbiotic, heterocystous, N2-fixing blue-green alga, isolated from the coralloid roots of a xerophytic plant,Cycas revoluta, grew best in liquid medium supplemented with 4 mM NO 3 . Morphologically, the isolated alga was identical to that of the natural endophyte but the cell size had decreased markedly. The alga was heterotrophic. Intact coralloid roots had nearly 4 to 5 times more nitrogenase activity compared with natural- and laboratory-grown agla but nitrate reductase was inducible in both the forms. Plasmid(s) were found in both algal forms.
Keywords:Coralloid roots  endophyte  heterocyst  nitrogenase  symbiosis
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